Mycoremediation of Benzo[a]Pyrene by Pleurotus ostreatus Isolated from Wayanad District in Kerala, India
نویسندگان
چکیده
Benzo[a]pyrene (BaP) is a ubiquitous environmentally significant compound which due to its recalcitrant nature is often referred as persistent bioaccumulative toxin. The present study was carried on to determine the degradation potentials of three natural isolates of the white rot fungi Pleurotus ostreatus towards BaP, of which isolate PO-3 showed promising results. The factors affecting biodegradation efficiency of the isolate in liquid culture were also investigated. An increase in the BaP concentration in the medium resulted in the decrease in the extent of degradation and the fungal biomass formation. Addition of co-substrates improved the degradation efficiency of the isolate and the highest degradation (52.3%) was mediated by glucose amendment in the media. Organic nitrogen sources proved to be superior nitrogen supplements and yeast extract supported the maximum utilization of BaP (57.32%). Inorganic nitrogen sources though supported the fungal growth, were insignificant in enhancing degradation. Surfactants improve the bioavailability of the otherwise recalcitrant compounds. Incorporation of Tween 80 facilitated an elevated level of BaP removal (62.14%) as compared to the control and other ionic and non ionic surfactants. Thus, Pleurotus ostreatus PO-3 can significantly degrade the toxic BaP with certain alterations in the biodegradation strategies and can be a potential candidate for the reclamation of sites polluted with polyaromatic hydrocarbon.
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Mycoremediation of Benzo[a]pyrene by Pleurotus ostreatus in the presence of heavy metals and mediators
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